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P#222

2’-HYDROXY-4’,5’-DIMETHYL-4-DIMETHYLAMINOCHALCONE, A NOVEL FLUORESCENT FLAVONOID WITH CAPACITY TO DETECT ALUMINIUM IN CELLS AND MODULATE ALZHEIMER’S DISEASE TARGETS

Fabiola Kamecki

  • CABA,
  • Argentina
  • Fabiola Kamecki ¹
  • , Carolina Marcucci ¹
  • , Mariela Ferreira-Gomes ¹
  • , Laureano Sabatier ²
  • , Damijan Knez ³
  • , Marina Rademacher ¹
  • , Alejandra Marcos ¹
  • , Felicitas de Tezanos Pinto ¹
  • , Luciana Gavernet ²
  • , Natalia Colettis ¹
  • , Mariel Marder ¹
  • 1 Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química y Fisicoquímica Biológicas Prof. Dr. Alejandro C. Paladini (IQUIFIB), Facultad de Farmacia y Bioquímica. Buenos Aires, Argentina.
  • 2 Universidad Nacional de La Plata, Facultad de Ciencias Exactas, Departamento de Ciencias Biológicas. Laboratorio de Investigación y Desarrollo de Bioactivos (LIDeB), Química Medicinal. La Plata, Buenos Aires, Argentina.
  • 3 University of Ljubljana, Faculty of Pharmacy, Ljubljana, Slovenia.

Alzheimer’s disease (AD) is a progressive multifactorial neurodegenerative disorder with several factors contributing to its aetiology such as abnormal protein aggregation (e.g., β-amyloid peptide), oxidative stress, alterations in neurotransmitter levels (e.g., acetylcholine, monoamines), ion metal accumulation in the brain (e.g., aluminium), among others. Due to the complex nature of this disease, there is a critical need to develop multitarget-directed compounds to address the different pathways involved.
In this context, the aim of this work was to synthesize a simple chalcone derivative with capacity to affect different key targets of AD neurodegeneration. Consequently, we report here a microwave-assisted synthesis of a new chalcone derivative, namely 2’-hydroxy-4’,5’-dimethyl-4-dimethylaminochalcone (1).
Compound 1 selectively chelated aluminium, inhibited the aggregation of Aβ1-42 peptide at 10µM (80.5 ± 6.3 %), behaved as a radical scavenger and inhibited acetylcholinesterase in vitro (IC50= 4.7 ± 1.1 μM). An extensive spectral study (UV/visible, fluorescence and MS) of chalcone 1/Al3+ complex as well as a molecular modelling study of its 3D conformation confirmed the structure of the complex (2:1). Additionally, chalcone 1 was capable of interacting with aluminium in cell lines HEK293T and SH-SY5Y.
In conclusion, chalcone 1 is a novel probe for the detection of Al3+ in cells and it can be regarded as a promising multifunctional ligand for AD treatment.

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